Rat IL-6 ELISA Kit (ER0004) is an ELISA kit with ultra sensitivity which can detect rat IL-6 up to 7.05pg/ml, it is suitable for research use
- Ready to use ELISA kit based on sandwich immunoassay.
- Total time required is around 80min.
- Highly specific to rat IL-6.
- Applicable for serum and plasma samples.
| Product | Rat IL-6 ELISA Kit |
|---|---|
| Product # | ER0004 |
| Category | ELISA Kits |
| Species | Rat |
| Unit Size | 96T |
| Storage | 2-8 degree Celsius. Room temperature with ice pack for shipment. |
| Format | Readily available commercial kit |
| Shelf-life | At least 12 months when stored properly. Opened kit shall be used in 30days. |
| Application | For testing of rat IL-6 in plasma, serum and/or other biological samples. |
| Application Notes | This product is only for research use. |
Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. It is encoded by the IL6 gene. In addition, osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. IL-6’s role as an anti-inflammatory myokine is mediated through its inhibitory effects on TNF-alpha and IL-1 and its activation of IL-1ra and IL-10. There is some early evidence that IL-6 can be used as an inflammatory marker for severe COVID-19 infection with poor prognosis, in the context of the wider coronavirus pandemic.
Rat IL-6 ELISA Kit Principle
This Rat IL-6 ELISA Kit is based on sandwich ELISA, the IL-6 specific monoclonal antibody was pre-coated in the microwells, the IL-6 in sample or the IL-6 standard will be captured by the coated antibody, after wash step, the unbonded IL-6 will be removed, and then another IL-6 specific monoclonal antibody is added and incubated. After then, the plate is washed again, and the substrate is added. The color of the wells is positively proportional to the IL-6 in it. After adding the stop solution, OD values are measured with ELISA reader. Unknown samples can be calculated from the standard curve.
Kit Components
This Rat IL-6 ELISA kit is a readily commercial product, it has the following components, which is sufficient for 42 testing in duplicated wells. Please always check the kit instruction manual for packaging details. For plasma and serum samples, the standard solutions are ready to use. For other samples, you may need to dilute the standard according to the kit instruction manual.
- Microtiter plate, 96wells,
- Standard, will be diluted to 7 vials,
- HRP conjugate, 1 vial,
- TMB substrate, 1 vial,
- Assay buffer, 1 vial,
- Wash buffer, 1 vial,
- Sealer film or plate cover, 1p pc
- Stop solution, 1 vial,
Specificity and sensitivity
The sensitivity is 0.26-0.43 pg/mL as MDD. The specificity is over 99%.
Result determination
The sample OD value is measured by an ELISA reader, then the standard curve is plotted. We recommend 4PL regression model or another equivalent model.
Storage and expiry
2-8 degree Celsius for at least 12 months.
Caution
- This kit is for research use only.
- The standard solution in the kit is Rat IL-6.
- Please read the kit instruction manual carefully before testing.
- Each run will take 80min or more, please schedule your time before experiment.