TGF-beta 1 ELISA Kit (EH0012) is an ELISA kit with ultra sensitivity which can detect mammal TGF-beta 1, including human, mouse, rat TGF-beta 1, up to 33.32 pg/ml, it is suitable for research use
- Ready to use ELISA kit based on sandwich immunoassay.
- Total time required is around 80min.
- Highly specific to mammal TNF-alpha.
- Applicable for serum and plasma samples.
| Product | TGF-beta 1 ELISA Kit |
|---|---|
| Product # | EH0012 |
| Category | ELISA Kits |
| Species | Rat, mouse, human, etc |
| Unit Size | 96T |
| Storage | 2-8 degree Celsius. Room temperature with ice pack for shipment. |
| Format | Readily available commercial kit |
| Shelf-life | At least 12 months when stored properly. Opened kit shall be used in 30days. |
| Application | For testing of rat TGF-beta 1 in plasma, serum and/or other biological samples. |
| Application Notes | This product is only for research use. |
Transforming growth factor-beta (TGF-β) is a multifunctional cytokine that performs a dual role as a tumor suppressor and tumor promoter during cancer progression. Among different ligands of the TGF-β family, TGF-β1 modulates most of its biological outcomes. Despite the abundant expression of TGF-β1 in the liver, steatosis to hepatocellular carcinoma (HCC) progression triggers elevated TGF-β1 levels, contributing to poor prognosis and survival.
TGF-beta 1 ELISA Kit Principle
This mammal TGF-beta 1 ELISA Kit is based on sandwich ELISA, the TGF-beta 1 specific monoclonal antibody was pre-coated in the microwells, the TGF-beta 1 in sample or the TGF-beta 1 standard will be captured by the coated antibody, after wash step, the unbonded TGF-beta 1 will be removed, and then another TGF-beta 1 specific monoclonal antibody is added and incubated. After then, the plate is washed again, and the substrate is added. The color of the wells is positively proportional to the TGF-beta 1 in it. After adding the stop solution, OD values are measured with ELISA reader. Unknown samples can be calculated from the standard curve.
Kit Components
This TGF-beta 1 ELISA kit is a readily commercial product, it has the following components, which is sufficient for 42 testing in duplicated wells. Please always check the kit instruction manual for packaging details.
- Microtiter plate, 96wells,
- Standard, will be diluted to 7 vials,
- HRP conjugate, 1 vial,
- TMB substrate, 1 vial,
- Assay buffer, 1 vial,
- Wash buffer, 1 vial,
- Sealer film or plate cover, 1p pc
- Stop solution, 1 vial,
Specificity and sensitivity
The sensitivity is 0.097 pg/mL as MDD. The specificity is over 99%.
Result determination
The sample OD value is measured by an ELISA reader, then the standard curve is plotted. We recommend 4PL regression model or another equivalent model.
Storage and expiry
2-8 degree Celsius for at least 12 months.
Caution
- This kit is for research use only.
- The standard solution in the kit is recombinant TGF-beta 1.
- Please read the kit instruction manual carefully before testing.
- Each run will take 80min or more, please schedule your time before experiment.